Intheyear1524,theformerAssassinEzioAuditoreretiredtoavillainthecountrysideofTuscany,withhiswifeSofiaandchildrenMarcelloandFlavia.WhileEziowroteinhisstudy,SofiaaskedhimtowatchFlaviawhilesheandMarcellovisithissisterClaudia.Shortlyafter,helefthisstudytotendtheirvineyardswhileFlaviaplayednearbythevilla.Inthemidstofhisharvest,hesawastrangercloakedinblack,talkingtoFlavia.Fearingtheworst,Eziohurriedlyinterceptedthestranger,thoughFlaviatoldhimthatshewasnodanger,asEziosawhernecklacewiththeAssassininsignia...
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